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dos.5. Ca dos+ imaging when you look at the neonatal OHCs The fresh rat Ocm gene (series for the Profile S01) was cloned towards pcDNA3.1CFP and you may pEGFP-N1 Banner (Addgene #60360). Following, EGFP-Flag try eliminated using BamHI (NEB) and NotI (NEB) and you can substituted for the mCherry series ...